Coding

Part:BBa_K3525000:Design

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_high-school   (2020-10-18)


ER sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 856
    Illegal BglII site found at 1353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1778
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First, we divided the lacZ coding sequence into two parts, the first 122 amino acids, and the last 123 amino acids.

Then, we divided the intein coding sequence into two parts, the first 206 amino acids, and the last 387 amino acids.

Most importantly, we combine the sequences in the following order: the first 122 amino acids of lacZ, the first 206 amino acids of the intein, the hera coding sequence, the last 123 amino acids of lacZ, and the last 387 amino acids of the intein.

Source

Rubing Liang, Jing Zhou. New Escherichia coli strain construction for environmental estrogens screen based on estrogen-sensitive intein. Journal of Biotechnology. Vol.136/S(2008)S1-S772 Jing Zhou, Rubing Liang, Wenhua Wang. New Escherichia coli strain construction for environmental estrogens screen based on estrogen-sensitive intein. Environmental Science and Technology 4th International Symposium on Novel Carbon Resource Sciences. 2009.

References